细胞培养基
Ham’s F-10
18Ham's F-10营养混合物（Ham's F-10 Nutrient Mixture）是由Ham在1963年针对CHO细胞无血清培养设计出的一种培养基，Ham's F-10包含丰富的营养成分，适合用于人二倍体细胞的培养，低血清条件下细胞的克隆化培养，哺乳动物细胞的正常培养，白细胞的染色体分析以及大...
查看全文RPMI-1640
RPMI-1640是Moore等人于1967年在美国纽约州法罗市的罗斯韦尔公园纪念研究所（Roswell Park Memorial Institute, RPMI）开发出来的，RPMI是该研究所开发的一类细胞培养基，1640是培养基代号。RPMI-1640是改进型的McCoy ‘s 5A培养基，使用碳酸氢盐缓冲系统，与大多数哺乳动物细胞培养基不同的是其典型的
PH8的配方。RPMI-1640培养基最初是为淋巴细胞培养专门设计的，现在已广泛应用于各种正常细胞和癌细胞的培养，包括 HeLa细胞、Jurkat 细胞、 MCF-7 细胞、PC12 细胞、PBMC 细胞、星形胶质细胞和癌细胞，是使用最为广泛的培养基之一。RPMI RPMI1640 培养基相比其他培养基之所以具有独特的效果，是因为其中含有还原剂谷胱甘肽和高浓度的维生素。RPMI 1640 培养基含有生物素、 维生素 B12 以及 PABA，这些成分是 Eagle’s MEM 和DMEM所不具备的。针对广泛的细胞培养应用，我们提供多种组分的 RPMI 1640 改良培养基。
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Modern Equipment
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Individual Approach
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DMEM培养基（Dulbecco’s Modified Eagle Medium）
12Dulbecco's 改良培养基--DMEM（Dulbecco's Modified EagleMedium）是在MEM培养基的基础上研制的，与MEM培养基相比，氨基酸的含量增加了2倍，维生素的含量增加了4倍，同时还增加了非必须氨基酸、微量铁离子以及丙酮酸钠。DMEM培养基最初设计葡萄糖含量为1000mg/L（...
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MEM培养基（Minimum Essential Medium）
13也称最低必需培养基、最小基本培养基或低限量Eagle培养基，由Harry Eagle在Eagle基本培养基（BEM）的基础上发展而来的，是一种最基本、适用范围最广的培养基，是动物细胞培养中最常用的培养基之一，包括包括HeLa、BHK-21、 293、 HEP-2、HT-1080MCF-7、 成纤维细...
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