IMDM（Iscove’s Modified Dulbecco Medium）

2021年3月25日news 120

IMDM即Iscove改良的DMEM培养基，是Guilber 和Iscove在1976年设计的，用以培养红细胞前体细胞和巨噬细胞。IMDM培养基在DMEM培养基的基础上添加了硒、HEPES、丙酮酸钠以及额外的氨基酸和维生素，营养非常丰富，非常适合于快速增殖，高密度细胞培养。IMDM培养基不仅可以培养有特殊养要求的细胞（如小鼠B淋巴细胞，LPS刺激的B细胞，骨髓造血细胞，T淋
巴细胞以及各种杂交瘤细胞），还可以作为一些独特的无血清培养基的基础液。针对广泛的细胞培养应用，我们提供多种组分的 IMDM改良培养基。

高糖型培养基普遍应用于生长快、粘附性低的细胞、杂交瘤的骨髓瘤细胞、克隆细胞、DNA转染的转化细胞、各种原代病毒宿主细胞、单一细胞的培养以及疫苗的生产，例如利用CHO细胞表达EPO和生产乙肝疫苗。低糖培养基更适合代谢作用较慢、依赖性贴壁细胞的培养。不含葡萄糖的培养基可以根据研究需要，随意调节葡萄糖的浓度，方便快捷。

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Modern Equipment

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Individual Approach

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